Monday, August 31, 2009

goodbye, and ola

read this last night in an astronomy book:
The curfew tolls the knell of parting day,
The lowing herd winds slowly o'er the lea,
The ploughman homeward plods his weary way,
And leaves the world to darkness and to me.
-Andrew Gray, Elegy written in a country churchyard

i'm leaving for the miami airport in less than an hour and will be arriving in curitiba, brazil early sunday morning. in the off chance you'd like to send me mail, send it here:
Brian Yates
Antioch Study Group
c/o Prof. Heitor de Andrade Neto
Rua Lamenha Lins, 2498 – Rebouças
CEP 80.220-080
Curitiba, PR BRAZIL

Wish me luck.

Sunday, July 26, 2009

Sunday, June 21, 2009

The Effects of Atrazine and Roundup on Freshwater Planktonic Communities

What follows is the proposal for my research project that I will be working on for the remainder of the summer at West Texas A&M University. I neglected to post this earlier so here it is... it helps to understand the situation greatly if you go to this website. Pictures of fish kills can be found here. This project aside from being really interesting will be incredibly helpful information to the state, severely reduce economic impacts of golden algae blooms, and if I'm published, put me on a straight path to graduate school.

ABSTRACT: Over 12 million fish have been killed in Texas since 1985 due to toxic golden algae (prymnesium parvum) outbreaks, resulting in huge losses to local economies. Although various investigations have been conducted on the conditions promoting blooms, the mechanism by which algal growth occurs is unclear. Most outbreaks occur in agricultural watersheds and coincide with the application of pre-emergent herbicides. We hypothesize that atrazine and Roundup inhibit the growth of obligate photosynthesizing organisms and cause a shift in the planktonic community to facultative aerobic organisms. This should correspond to a statistically significant difference in population diversity and relative abundance of all species. The population of p. parvum in the aerobic state should increase and produce toxic metabolites.

Procedure: We will randomly collect water samples from nearby Lake Tanglewood, which has experienced fish kills due to golden algae outbreaks. Aquaria with a half gallon of water will be inoculated with water samples and aerated constantly using an air pump. Aquaria will be subjected to 12 hour photoperiods. Population diversity and relative abundance will be measured frequently over 3 days or more under atrazine concentrations of 0 (control), 0.1, 1, and 10 ppb while populations will be assessed under Roundup levels of 0.1, 1, 10, and 100 ppb.

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I know that scientific writing is oftentimes a bit too succinct and technical, so the idea for this project comes from a problem occurring in Texas: golden algae blooms. During January-March, the water in some places turns golden brown and thousands of dead fish rise to the surface. The state of Texas will then come in and clean everything up, but it will take a couple of months for an area to recover from a bloom like that and for fish to come back and repopulate. This is caused by golden algae (latin name prymnesium parvum) which multiplies for unknown reasons. When it does this it gives off a toxin to fish which are very sensitive because they have gills. Molluscs and shellfish tend to die off too, and no one knows why this is happening.

We believe this is occurring because atrazine, an herbicide, is running off into the surface water and causing this algal bloom. Golden algae is a "mixotroph", which means it survives normally by getting light energy and conducting photosynthesis, where it produces oxygen as a byproduct. When it is in this state it causes no harm. However, the alga can revert to a "heterotrophic state", in which it consumes other organic nutrients (lipids or fats, amino acids, bacteria) for food. When it does this, it gives off toxic metabolites to catch and digest its food, and that is when the poisons are given off.

Atrazine causes this reaction by inhibiting photosystem II, which is the first step in photosynthesis. When the alga cannot photosynthesize, it goes into its heterotrophic state, where it consumes other nutrients and bacteria for food, and gives off these toxins. There is a lot of atrazine runoff from agricultural lands, and most fish kills happen in the watersheds of these farms. If we find that atrazine does cause the golden alga to give off toxins and kill other plankton, farmers may need to establish corridors between their farms and watersheds which will reduce atrazine runoff (introduce native plants to narrow strip of land which will uptake the pesticide and reduce pollution).

So for my experiment, I'm going to a lake which has experienced a bloom to sample water and collect plankton. I'll combine all the water, mix it up with a plankton, and then put it all into a bunch of different aquaria. In half of the aquaria I'll have different concentrations of atrazine, and in half I'll have different concentrations of Roundup. Then I'll have a few aquariums which have no herbicides, this is my "control", and I'll compare the effects of the varying concentrations of atrazine and Roundup to that of the aquariums with no herbicides.

From this information I should be able to establish a "dose-response curve", this is a graph which shows the dose of the herbicide on the x-axis and the response on the y-axis. My response variables (what I'm testing for) will be the relative abundance of the algae (how much golden algae is there compared to the other types of algae) and population diversity (low population diversity = just a few species of algae, high population diversity = many different species). We predict that the relative abundance of golden algae will increase with increasing levels of either herbicide, while population diversity will decrease.

With the dose response curve, regulatory agencies such as the EPA or Texas Department of Parks and Wildlife/Texas Council on Environmental Quality should be able to establish acceptable levels of atrazine and Roundup in waters which are sensitive to golden algae outbreaks. We may reccomend a NOAEL, or "no observable effects level", the level of herbicide in the water (i.e. 10 parts per billion) at which we see no effect whatsoever on the planktonic community. This information should help these agencies to monitor outbreaks and areas which are particularly vulnerable to fish kills. In addition, this will make regulatory agencies increasingly aware of the ecological nature of risk assessment and toxicology.

In toxicology, we frequently test the effects of a certain chemical or pesticide on a target species, such as fathead minnows or rats (in the case of carcinogens). However we don't really take ecology into account. For example in this study, atrazine is not normally toxic to fish. However, atrazine kills off phytoplankton and causes golden algae to give off poisons which are toxic to fish. So, atrazine turns out to be indirectly toxic to fish. When atrazine was being tested for the public market, no one thought to test it in an ecosystem or on algae of any kind, because what does it matter if an herbicide kills off algae? That's just less scum on the surface of most ponds and lakes, right?

In certain cases, that logic may be true. But increasingly, we're finding out that it's important to study the effects on the entire ecosystem. If an herbicide runs off into surface water and kills off all phytoplankton, well, what are the zooplankton going to eat? And then if the zooplankton die, what will smaller fish eat? And larger fish, once those fish die? So what happens is we have "trophic-level effects" on a large scale, which, if we don't test for, will happen in the real world. Algal blooms, fish kills, problems with invasive species, all can be the products of our limited ability or desire to study the effects of toxins on a species without realizing the relationships that species has with its food web and surrounding environment.

Monday, June 8, 2009

Weekend in New Mexico

So a few hours ago we all got back from a weekend trip through the mountains of New Mexico, which was pretty awesome. We got to camp out under the stars two nights straight, saw the city of Taos, and got to hike around the rim of a volcano. I'll start from the beginning...


We left Friday morning from WT around 10 AM and loaded all 9 of us plus Ray into the van, hitting the road pretty quick. For a few hours we watched the flat landscape of crops and cattle ranches turn into rolling hills of shortgrass prairies, saw the soil turn a deep red and not long after crossing into NM out of nowhere we see huge mesas pop out of the ground (mesa, spanish for 'table' due to its vertical slope and flat tops, were formed millions of years ago by rivers of lava flowing through and pooling in sedimentary rock which eventually eroded away to leave only the volcanic rock). we began seeing for miles around us an arid, rugged scrubland of mesquite, pinyon pine, and juniper, and from time to time a pronghorn antelope (the pronghorn is common in the southwest and as one of the western hemisphere's fastest land mammals can reach 70 mph - it actually keeps its mouth open to get more oxygen as it runs). Although mesquite has been derided by many ranchers for absorbing much-needed water and spreading over rangeland rapidly, it provides excellent soil stabilization and acts as a natural fertilizer by fixing nitrogen close to the roots, providing habitat for better quality grass species. 


We drove for miles through the plains, watching the landscape become more rugged as more and more mesas popped up - "mesa country" is what they called it. Eventually we got to the Conchas Dam/State Park where we stopped for lunch. The reservoir was extremely low and there was no outflow whatsoever. The shrubs growing in the "river" bottom near the base of the dam indicated that water hadn't been let out of the dam in quite some time. After some bagels with lunch meats we played frisbee and walked around a bit, but besides some crows and small lizards scurrying about the brush we didn't see much wildlife. Back on course to Las Vegas, NM (not Nevada).


We begin climbing in elevation significantly - we begin at about 5,000 feet and just keep going up from there. In the distance, snow-capped mountains appear and the ecosystem around us is changing. We begin seeing larger shrubs, some deciduous trees and smaller pines. As elevation increases, climate cools and moisture increases, the soil substrate can support a different subset of trees and shrubs which provide habitat for a different variety of animals. As we begin winding our way through hairpin turns we see broadtail hawks, ravens, mule deer, and ponderosa pines. The air is considerably cooler as we get out and stop near the top of a large ravine for a photo op. We spot some hawks and a whiptail and after throwing some rocks and stuff down the canyon wall and move on. 


After a few more photo ops at nameless mountain ridges overlooking beautiful valleys which neither words or pictures can entirely capture, we reach about 9500 feet and pull off onto a mountain road which winds upward, following a pristine montane stream. Surrounding us are huge pine trees reaching 60-70 feet and small meadows through which the babbling stream meanders. We drive for about 15 minutes before finding a suitable campsite away from other campers hauling ATVs and noisy generators. 

We begin unpacking the van and cross the stream into a meadow to gather firewood. After hacking down a number of branches and feeling good about the amount of wood I've gathered, we see Brooke (another REU) carrying a 10-12 foot dead tree trunk over her shoulders like it was nothing. After getting a fire going, Ray starts cooking pasta. Zach has already wandered off in search of scorpions and tarantulas while Kyle, Brooke and I ascend the 55-60 degree angle slope for a short hike before dinner's ready. They go off to the right, while I stay left and follow a muddy creek which runs straight down the slope, which may have been a mistake because I muddied up my boots pretty bad on the way back. Within 5 minutes I'm out of breath and hanging onto a rocky outcrop. 10 minutes later I get to a larger boulder and get a pretty good view of the surrounding area - towering pine trees outlining steep valleys for as far as I can see. I descend and we eat dinner. Afterwards we wash dishes; Ray, Kyle, Brooke, Marty and I all go out for a short nature hike. Brooke identifies a number of plants; Ray, Kyle and I only spot an American Robin, broad-tailed humminbird, and pine siskin. Later on we see a beaver dam at which a shallow pool is formed where small trout were jumping (if only I had my fishing pole!). On the way back we see, interestingly enough, a small white dog on the trail which runs up the side of the mountain and doesn't respond to our calls - we assume it had gotten separated from its owner. When we get back we make s'mores and throw around some glow sticks in the dark for fun. The moon eventually rises and brightens the area, so we put our mats down, get in the sleeping bags and fall asleep quickly listening to the bubbling stream under a starry sky.


Next morning, the beautiful mountain scenery and bright sun on my face makes up for the fact that I slept on a pretty uncomfortable surface (an inflatable mat can only do so much). After some hot cocoa and pop tarts we pack up and move on to Taos. 


Before arriving we stop for a photo op on the other side of the mountains we camped in the previous night. We descend from the mountains and get back into the plains and mesa country. Eventually we arrive at Taos and explore a local farmers market, which offers some organic vegetables and dairy products, various legumes and indian corn while some local musicians play huge xylophones and sing some sort of soothing tribal-esque blithe song. We walk on through the town, which is a hilly landscape of colorful gardens amidst modern adobe homes adorned with bright wooden trim. Street vendors sell chili peppers while locals seem to walk around without a care in the world with tie-dyed shirts. The 400-year-old Spanish plaza has a flowery gazebo with a few statues surrounded by shops selling jewelry, paintings, and native american crafts. 


We walk on to get lunch and on the uphill climb traffic stops as an apparent hippie parade marches through. Painted buses and old VW bugs cruise by blasting 60s-era songs while people between 14 and 70 wave colorful flags protesting for peace and commemorating the 40th anniversary of woodstock. while browsing through the pictures i actually put on "get together" by the youngbloods because it reminded me a lot of the ending scene of "fear and loathing in las vegas" and many other late 60s/vietnam war era movies. i know it's not my cultural scene at all but it was quite interesting to see so many people who were still living in that moment over 40 years later, and taos definitely seemed to be the place for them. i read somewhere that about 75% of Taos's population is made up of visual artists, and if you include musicians and cinematographers it almost reaches 100%. 


Fortunately, a smaller percentage of the population makes incredibly good Tex-Mex, specifically the folks down at Michael's kitchen cafe and bakery. Ray and I ordered the stuffed sopapillas, while Eric ordered (though having trouble pronouncing) flautas, jenny got tacos i believe and kristen some incredibly spicy green-chile beans. with lunch we got a basket of huge palm-sized white fluffy sopapillas, onto which you spread an interesting honey butter. the portions were huge, and after devouring the delicious pile of thick sopapilla bread, meat, cheese, lettuce, tomato, guacomole, onion, green chile sauce and sour cream, i was ready to take a nap somewhere. 


afterwards we wandered down the paseo del pueblo, checking out the myriad species of flowers planted in front of ornamental clay and metal sculptures of all types, passing gardens in front of cobblestone walkways to adobe houses with s-style red clay roofing - i realize i love everything about southwest architecture. we get back to the main plaza; kristen goes off to find a blanket while i wander around and take some photos of the general plaza area. it reminded me very much of spain, but more mountainous and cleaner - there wasn't a bar in sight, now that i think of it. finally we head on back to the van and make our way north to the mountains.


leaving taos we first cross the rio grande gorge bridge and stop to appreciate it. the cantilever truss bridge built over 40 years ago hovers an amazing 650 feet over the rio grande, which appears as a small brown trickle at the bottom of an immense crack in the earth. the sheer height and grandeur of the bridge and the surrounding mountainous landscape just puts you in awe, and your forced to grab the railing as cars zip past at over 65 mph only a few feet from where you're walking. we spot a female bighorn sheep and a few young walking maybe 200 feet from the bottom of the gorge, quickly climbing over rocks to move farther downstream. birds soar from beneath the cliffs upward and catch the headwinds at the top of the gorge, float for a quick second, and shoot like a bullet back down to the base of the gorge. i think i got the gorge from every angle in every possible mode but still found it hard to fully convey the natural beauty of the place. 


finally we get back on track toward the mountains and i fall asleep, only to wake up to rolling hills of shortgrass prairie and pristine lakes surrounded by forested mountains. we climb another mountain range and descend slightly into a valley, following a stream which winds through a campground where unfortunately all the sites were occupied. so we zip past and an hour later come upon sugarite canyon state park. ray stops the van to talk to an old-timer working at one of the campgrounds who tells us to go on ahead but warns us of black bear citings in the area. we move northward and climb switchback turns to reach campgrounds on the side of a mountain with beautiful views of the surrounding valley. above us is shrub-covered mountain ridges with red caprock jutting out, just asking to be hiked.


after gathering some firewood, ray fires up the stove to cook taco meat and we all fill our plates with meat, beans, lettuce, onion, sour cream and mild sauce. it didn't compare to what we had for lunch, but we were all hungry so i don't think anyone cared that much. in order to finish our hike before sundown we decided it'd be best to start right after dinner, so we did. 


we began on the 'scenic vista' trail, which we hoped would bring us to the very top of the ridge, and luckily we were not disappointed. an interpretive map explained botanicals such as mountain mahogany, juniper and pinyon pine, while some sites explained lichens and the value of fire to the ecosystem (which seems to be a common occurrence everywhere in the arid southwest). a lichen is a symbiotic relationship between an alga and a fungi, in which the alga photosynthesizes to provide food for the fungi, which provides structural support for the alga. strangely, they have a sort of dirty yellow lichen here as well as the light green - while in new jersey i've only seen the light green type. 


eric, marty and i wandered off the trail to climb some boulders, and only after we reached the top did we realize that there was too much vegetation and climb through to ascend and we would have to go back. after returning to the trail, we climbed the increasingly-steep rocky path and finally, after clambering up a few last monster rocks, we reached the top - the mountain ridge. 


it was amazing - looking back to where we came from, we saw the entire forested valley and opposing mountain ridge, looking southward the valley widened until it separated and opened up into mesa-filled plains country. the shadow of our ridge from the slowly sinking sun encapsulated the majority of the valley in front of us, and we turned around to face the other side. instead of seeing another valley on the other side, we saw only an immense, flat plain of treeless shortgrass prairie. it was like we had reached the next level - and at about 8600 feet in elevation, where no road or sign of civilization existed, we began walking across this plain to find its edge. in the distance we saw another mountain rising from this plain - if we had time we would have attempted that one, for sure. unfortunately after walking about 20 minutes we realized this plain seemingly had no end, and we would have to return before the sun went down unless we wanted to descend in darkness. so we did.


that night we camped out again under the stars, this time without any tall pine trees enshrouding our view in the least. right before bed we explored a nearby montane marsh, spotted a mule deer, kyle got out a sonar device looking for bats (we didn't find any), and ray spotted a huge owl (couldn't ID it though).


i woke up the next morning to the smell of coffee roasting and the view of red rock canyon walls nearly covered in pines and shrubs, with the valley below us glistening in the sunlight. the beauty was absolutely indescribable. 


we set off for capulin volcano, a million year old volcano which erupted more than a few times during the past several thousand years. we took the road that encircled the thousand-foot high monster to the mouth of the crater and began the hike around the volcano's rim. it was incredibly windy around the rim, and i nearly blew off a rocky outcrop while doing a dumb pose for my picture (which i probably deserved). small signs around the trail explained the significance of the plants, animals, and most importantly geology of the area. we found a variety of seemingly different rocks on our hike. which were made of the same material but formed in different ways depending on how the molten rock was shot straight out of the volcano or bubbled over the mouth in the lava flow. it was really hard to get a good shot of the crater because of its whole 3-D nature and ginormous size, but i tried. We could see for about 30 miles from the top, but the views didn't beat the ones from camping the previous night. 


on the way back home we counted over 300 pronghorn antelope. that was about it. it was a fun trip. the next 7-8 weeks will probably not be nearly as exciting, but exciting or not, there's still much more in store. 


My pictures from the weekend can be seen here: http://profile.imageshack.us/user/byates/images


Thursday, June 4, 2009

First week of REU

Hey guys, here's the basic summary for the first week of the Research Experience for Undergraduates at West Texas A&M University (okay it's pretty long, so not so basic - sorry about that).

First week of REU - journal


The first week my REU at West Texas A&M University (WTAMU) has been an amazing experience. I was picked up by Dr. Ray Matlack, the program coordinator at the Amarrillo airport, who gave me extensive background on the wildlife ecology of the area. He introduced me to the concept of playas, shallow depressional wetlands that periodically form after rainfall and serve as a rudimentary form of drainage. We stopped along the way to check out a prairie dog colony, where we saw the small animals and some burrowing owls. After dropping me off and getting me signed into the dorm, I met the other REUs, who are all from different parts (New Jersey, New York, Pennsylvania, Wisconsin, Salt Lake City, Chicago, and Iowa). Everyone is excited to be here and interested in ecology and environmental science. Later on in the day we walked out to a prairie dog colony and found some tarantula burrows. 

That next day we were introduced to the rest of the participants, filled out some paperwork, and met our research mentors. The dean of the college of agricultural and environmental science welcomed us and introduced us to the geography of the Texas high plains. Afterwards, we met our professor for the one-credit hour course we would be taking named "Ecology and Environment of the Southern High Plains". The class sounds pretty exciting; we were given "Caprock Canyonlands" by Dan Flores as well as some other literature to read for next week. 

Afterwards I got to discuss my research plans with my mentor, Dr. Jim Rogers, director of the environmental science program at WTAMU. Dr. Rogers has been in the field of ecological risk assessment since 1987 and is a huge wealth of information on anything you want to know related to the field. This summer I will be examining the impacts of atrazine and Roundup on golden algae. This study will hopefully end up being published in SEATAC and allow land managers to provide for buffer strips between agricultural land and surface waters to allow the pesticides to settle out.  

After dinner with the participants and mentors, we all drove out to Palo Duro canyon. The site is amazing; after driving for 20 minutes and seeing only flat land, a huge crack in the earth appears, which eventually widens to become a huge rocky landscape of red-yellow-brown canyon walls, towering rocky outcrops, and lush vegetation at the canyon bottom. Needless to say all of our jaws dropped at the site of this natural beauty. We took the hairpin turns and braved a thunderstorm to reach the bottom of the canyon, navigating our way through the rugged landscape. 

One of the interesting things about this bioregion is that the ecology and wildlife is different depending on which part of the canyon you are in. South-facing canyon ridges are typically drier due to the position of the sun and support wildlife typical of xeric ecosystems, whereas the north-facing side supports more lush vegetation and mesic systems. As I read more in the book I will update this in regards to different microhabitats, the climate, etc.

After the trip to Palo Duro, we all got back to the dorms and hung out for a while. I left with Zach, another REU extremely interested in arachnids, to go out to the field and look for some tarantulas. While blacklighting for the hard-to-find nests we found a large female bufo woodhousii, woodhouse toad, a common toad native to the mid- and southwestern states. Afterwards we found some tarantula burrows and Zach poured some water in the hole to get it to come out. They really are amazing creatures close up; they can take over 10 years to reach full maturity because of their slow metabolism, but I think their sit-and-wait tactics for obtaining prey is pretty cool. Zach also showed me a video of one of the creatures brushing the urticating hairs of its rear abdomen with its legs while partly in the hole; this is an anti-predator defense which pushes urticating hairs in the direction of the predator and induces physical irritation. The hairs lodge in the animal's skin or eyes and can be quite uncomfortable, according to Zach's experiences with the animals.

On the way back from the field Zach talked to me about the taxonomy of different arachnids and we discussed some different ideas in evolution and projects we had worked on in the past. It's great to be able to participate with people who are interested in various scientific concepts and genuinely enjoy the process of doing science.

On Tuesday, Ray gave us his personal introduction to the biology of the Southern High Plains. We talked about a number of things; one of which was the rain shadow which causes the aridity of this area. When winds from California blowing east come to the Rocky Mountains, the air is forced up to the top of the mountains. As the warm air at the bottom cools down, it condenses and falls as precipitation on the west side of the mountains. The air that blows over the mountains, then, is very much devoid of moisture. Our location in Canyon in the rain shadow of the mountains, combined with high winds causes us to be very dry and have a panevaporation rate of over 6 feet per year. Dr. Rogers was telling me the record for panevaporation was half an inch in only a day. 

Our high winds and unique location allow for huge fluctuations in temperature. Changes of 40 degrees have been known to occur within a period of a few minutes. Low-pressure zones can quickly arise and stop otherwise sunny, cloudless skies in a matter of a few minutes. Rainfall, however, is intermittent, though when it does occur flash floods can flood roadways and playas. On our trip to Palo Duro we also witnessed cars stuck under overpasses to wait out the thunderstorm for fear of hail damage. 

Fire is a common occurrence in the high plain bioregion, though nowhere near as common as it was in the undisturbed system. These occur naturally every 5-10 years and typically burn up low-lying vegetation because it burns most quickly, while hardwood trees, typically absent except where humans have planted them, are slower to catch on fire. 

While the red river forms the base of Palo Duro Canyon and eventually flows eastward to form the Texas-Oklahoma border, the Canadian river lies to the north. This 96 mile river, whose headwaters lie in the Sangre de Cristo mountains of New Mexico, flow into huge reservoirs from which large populations of high plains residents derive their drinking water. Ute Lake and Conchas lake are both reservoirs of the river in New Mexico, where high demand for water has caused water levels to drop significantly and reduce flow eastward. Downstream, Lake Meredith in Texas has been affected by the upstream reservoirs because it doesn't receive nearly as much water as it should. Consequently, this reservoir serves as a water source for countless agricultural operations. Indeed, 95% of the water used in this area goes toward agriculture, mostly for cotton, wheat, corn, and sorghum. 

The other agricultural operations here largely involve cattle ranching. This makes up a huge amount of income for the area, and Ray estimates that approximately 1/3 of all beef produced in the United States comes from a circle forming 100 miles around Amarrillo. Hereford, which is south of Canyon, is known as the beef capital of the world, while Amarrillo houses one of the largest meat packaging plants in the entire United States.

Anyway after Ray taught us all of this information plus some more, a woman from communications came by and we took a self-assessment based on our personalities. She divided us up into four groups (blue, gold, green, and orange) based on how we answered these questions and helped us all to learn about each other. Afterwards we met with Ray and talked about what type of food we would eat while camping in New Mexico over the weekend and went out to eat. We ate at this amazing Mexican restaurant where I got a taco with grilled chicken, grilled pineapple, and grilled onion (thanks Ray for the recommendation). I'm excited to try the different types of Mexican food they have down here - there's more variety than the typical once-in-a-while Mexican place you find in New Jersey and Taco Bell. It's also incredibly cheap - all the sit-down restaurants we've been to so far have had specials for no more than $5 or $7 with a soda. Definitely a lot more than you would spend at most places in New Jersey. 

Later on we talked with our mentors a while longer and finally went out with Ray to get dinner at a local burger place. Afterwards we had the opportunity to do some shopping at the nearby bookstore. I came back to my room, did some reading and went to bed. 

Wednesday (yesterday), Dr. Rogers was out for the day but I got to see how a scanning electron microscope worked. I met with Chris, one of Dr. Rogers' research assistants, and we went over to the engineering building to check it out. It was only there for a couple of days so we made use of it by checking out some pollen samples. It was amazing; the machine was very user-friendly and we got to see some really detailed, high-quality images of pollen grains. Each species of pollen grain was different. I'm later going to touch the pictures up in photoshop to show the true colors. Later, Dr. Ghosh, a biologist from the agricultural and environmental sciences department, came by and helped us identify the pollen grains further. He mentioned that from the structure of the pollen grain, we could tell which ones would be most irritating to people with allergies, then people surveying fields could tell from the abundance of plants whether there would be a high risk of allergies. 

We also collected a couple of insects and looked at them under the microscope. It was amazing seeing these guys really close up, the detailed anatomy was so apparent it just put you on a whole other level. Most of the time we conduct experiments on small organisms and don't really get to observe them except with our eyes, but being able to see these organisms on such a high scale and resolution was just stunning.

After lunch at a burger place (which had an amazing virgin drink called a cherry limeade) we returned and helped Chris bring some soil to the greenhouse where he would plant beets for an experiment. I'm not going to go into details, but he's figuring out a way to maximize water retention in beets so they can be grown in more arid areas and reduce water application. The multitude of projects going on here is just astounding. 

That reminds me, I haven't talked about the other participants projects. Zach is conducting an inventory on the different species of scorpions and tarantulas in this bioregion because the taxa are exremely understudied; he'll also be examining the phylogeny and determining whether changes should be made based on DNA studies. My roommate Marty (Iowa State) and Eric (Stockton College) are doing inventory on bird nests at Crossbar Ranch - they'll be spending Wednesday-Sunday in the field to determine the distribution of various bird nesting sites. 

Kyle (Catawba College) is working under Dr. Matlack on a project involving echolocation in bats and whether this ability is used to find habitat in canyon walls. Kristen (SUNY Binghamton) is investigating cell differentiation in onion cells as well as the effects of dieldrin on the plant. Brooke (Spring Arbor) is looking at volatile organic compounds (VOCs) coming from concentrated animal feeding operations (CAFOs). Jenny (Loyola) was going to be looking at the effects of euthanizing chemicals in horses on ground and surface water but the testing may turn out to be too expensive, so I'll have to get updated on what her project's going to be.

Yesterday after finishing up with the SEM I went home and check out the gym, did some reading until late and fell asleep.

Today Dr. Rogers returned to the office so we talked about the idea for the proposal, only after I showed Zach the scanning electron microscope. He got some great pictures of scorpion claws at 3,000x.

I waited for a while to talk to Dr. Rogers because he was busy dealing with someone from the Ecological Rights Foundation on PCB pollution in the San Francisco Bay. They needed his assistance compiling a TMDL document, but he's had some problems with it because both they created separate documents for dioxins and furans/PCBs, while the two are congeners. This means that the chemicals act in very similar manners, and the effects of both on an organism would be additive. For example, assuming a benchmark of 10 ppt: if both are assessed separately and the concentration of PCBs/furans turns out to be 9.7 ppt in surface water, while the concentrations of dioxins turns out to be 8 ppt, they would both still be within the benchmark. However both act similarly and the actual concentration of these chemicals together would be around 17 ppt, much higher than the benchmark for either chemical. So the bottom line is that in ecological risk assessment it is important to assess chemicals which behave similarly as having additive effects. Many chemicals do not have additive effects, in fact some chemicals reduce the toxicity of the other. More on that later.

So we developed our research plan in the morning, which I'll write up tonight, and we hammered out a budget of about $415 to spend on aquaria, tubing, bubblers, and filters. 

Tomorrow I'm leaving at 10 AM to leave for the mountains of New Mexico. We'll camp in the mountains for two nights at different sites and visit the city of Taos, a city with a rich Native American culture. I'll bring the camera along and hopefully get these pictures loaded up ASAP.